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flow cytometry bead array flex kit  (Becton Dickinson)

 
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    Becton Dickinson flow cytometry bead array flex kit
    Expression of cytokines and their mRNA in nasal mucosa of mice in each group (A) – (D) The mRNA expressions levels of cytokines in nasal mucosa which were determined by real-time PCR. After total RNA was extracted, it was reverse transcribed into cDNA, and then real-time PCR was performed to obtain the Ct value of each gene. We used 2 −ΔΔCt to calculate the relative expressions. β -actin was used as the internal control. ΔΔCt = [ΔCt (Ct value of target gene in experiment group-Ct value of β -actin)-ΔCt (Ct value of target gene in control group- Ct value of β -actin)]. Each group repeats five independent samples, and each independent sample repeats three times to take the mean value. One way ANOVA/Tukey was used for statistical analysis. All results were mean ± SEM. * p < 0.05. (E) – (H) The protein expression of IL-4, IL-5, IL-13, and IL-9 in nasal mucosa of each group which was detected by flow <t>cytometry</t> bead array. Each group repeats five independent samples. One way ANOVA/Tukey was used for statistical analysis. All results were mean ± SEM. * p < 0.05.
    Flow Cytometry Bead Array Flex Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flow+cytometry+bead+array+flex+kit/flow+cytometry+bead+array+flex+kit/pmc09510626-97-1-7
    Average 90 stars, based on 1 article reviews
    flow cytometry bead array flex kit - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "IL-9 neutralizing antibody suppresses allergic inflammation in ovalbumin-induced allergic rhinitis mouse model"

    Article Title: IL-9 neutralizing antibody suppresses allergic inflammation in ovalbumin-induced allergic rhinitis mouse model

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2022.935943

    Expression of cytokines and their mRNA in nasal mucosa of mice in each group (A) – (D) The mRNA expressions levels of cytokines in nasal mucosa which were determined by real-time PCR. After total RNA was extracted, it was reverse transcribed into cDNA, and then real-time PCR was performed to obtain the Ct value of each gene. We used 2 −ΔΔCt to calculate the relative expressions. β -actin was used as the internal control. ΔΔCt = [ΔCt (Ct value of target gene in experiment group-Ct value of β -actin)-ΔCt (Ct value of target gene in control group- Ct value of β -actin)]. Each group repeats five independent samples, and each independent sample repeats three times to take the mean value. One way ANOVA/Tukey was used for statistical analysis. All results were mean ± SEM. * p < 0.05. (E) – (H) The protein expression of IL-4, IL-5, IL-13, and IL-9 in nasal mucosa of each group which was detected by flow cytometry bead array. Each group repeats five independent samples. One way ANOVA/Tukey was used for statistical analysis. All results were mean ± SEM. * p < 0.05.
    Figure Legend Snippet: Expression of cytokines and their mRNA in nasal mucosa of mice in each group (A) – (D) The mRNA expressions levels of cytokines in nasal mucosa which were determined by real-time PCR. After total RNA was extracted, it was reverse transcribed into cDNA, and then real-time PCR was performed to obtain the Ct value of each gene. We used 2 −ΔΔCt to calculate the relative expressions. β -actin was used as the internal control. ΔΔCt = [ΔCt (Ct value of target gene in experiment group-Ct value of β -actin)-ΔCt (Ct value of target gene in control group- Ct value of β -actin)]. Each group repeats five independent samples, and each independent sample repeats three times to take the mean value. One way ANOVA/Tukey was used for statistical analysis. All results were mean ± SEM. * p < 0.05. (E) – (H) The protein expression of IL-4, IL-5, IL-13, and IL-9 in nasal mucosa of each group which was detected by flow cytometry bead array. Each group repeats five independent samples. One way ANOVA/Tukey was used for statistical analysis. All results were mean ± SEM. * p < 0.05.

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Flow Cytometry

    Schematic diagram and statistical diagram of flow cytometry. Figure (A) – (C) shows the Th2 delineation strategy of the control group, and other groups follow this scheme. Figure (D) shows the average number of Th2 cells in each group ( n = 5). One way ANOVA/Tukey was used for statistical analysis. All results were mean ± SEM. * p < 0.05.
    Figure Legend Snippet: Schematic diagram and statistical diagram of flow cytometry. Figure (A) – (C) shows the Th2 delineation strategy of the control group, and other groups follow this scheme. Figure (D) shows the average number of Th2 cells in each group ( n = 5). One way ANOVA/Tukey was used for statistical analysis. All results were mean ± SEM. * p < 0.05.

    Techniques Used: Flow Cytometry

    Related Articles

    Flow Cytometry:

    Article Title: IL-9 neutralizing antibody suppresses allergic inflammation in ovalbumin-induced allergic rhinitis mouse model
    Article Snippet: .. The flow cytometry bead array flex Kit (BD Biosciences, San Diego, California, United States) was used according to the manufacturer’s instructions to detect IL-4, IL-5, IL-9, and IL-13 levels in the supernatant. .. Total protein was extracted from the mucosa of the remaining five mice in each group using RIPA lysing buffer (Beyotime, Shanghai, China).



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    flow cytometry bead array flex kit  (Becton Dickinson)
    90
    Becton Dickinson flow cytometry bead array flex kit
    Expression of cytokines and their mRNA in nasal mucosa of mice in each group (A) – (D) The mRNA expressions levels of cytokines in nasal mucosa which were determined by real-time PCR. After total RNA was extracted, it was reverse transcribed into cDNA, and then real-time PCR was performed to obtain the Ct value of each gene. We used 2 −ΔΔCt to calculate the relative expressions. β -actin was used as the internal control. ΔΔCt = [ΔCt (Ct value of target gene in experiment group-Ct value of β -actin)-ΔCt (Ct value of target gene in control group- Ct value of β -actin)]. Each group repeats five independent samples, and each independent sample repeats three times to take the mean value. One way ANOVA/Tukey was used for statistical analysis. All results were mean ± SEM. * p < 0.05. (E) – (H) The protein expression of IL-4, IL-5, IL-13, and IL-9 in nasal mucosa of each group which was detected by flow <t>cytometry</t> bead array. Each group repeats five independent samples. One way ANOVA/Tukey was used for statistical analysis. All results were mean ± SEM. * p < 0.05.
    Flow Cytometry Bead Array Flex Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flow+cytometry+bead+array+flex+kit/flow+cytometry+bead+array+flex+kit/pmc09510626-97-1-7
    Average 90 stars, based on 1 article reviews
    flow cytometry bead array flex kit - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

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    Expression of cytokines and their mRNA in nasal mucosa of mice in each group (A) – (D) The mRNA expressions levels of cytokines in nasal mucosa which were determined by real-time PCR. After total RNA was extracted, it was reverse transcribed into cDNA, and then real-time PCR was performed to obtain the Ct value of each gene. We used 2 −ΔΔCt to calculate the relative expressions. β -actin was used as the internal control. ΔΔCt = [ΔCt (Ct value of target gene in experiment group-Ct value of β -actin)-ΔCt (Ct value of target gene in control group- Ct value of β -actin)]. Each group repeats five independent samples, and each independent sample repeats three times to take the mean value. One way ANOVA/Tukey was used for statistical analysis. All results were mean ± SEM. * p < 0.05. (E) – (H) The protein expression of IL-4, IL-5, IL-13, and IL-9 in nasal mucosa of each group which was detected by flow cytometry bead array. Each group repeats five independent samples. One way ANOVA/Tukey was used for statistical analysis. All results were mean ± SEM. * p < 0.05.

    Journal: Frontiers in Pharmacology

    Article Title: IL-9 neutralizing antibody suppresses allergic inflammation in ovalbumin-induced allergic rhinitis mouse model

    doi: 10.3389/fphar.2022.935943

    Figure Lengend Snippet: Expression of cytokines and their mRNA in nasal mucosa of mice in each group (A) – (D) The mRNA expressions levels of cytokines in nasal mucosa which were determined by real-time PCR. After total RNA was extracted, it was reverse transcribed into cDNA, and then real-time PCR was performed to obtain the Ct value of each gene. We used 2 −ΔΔCt to calculate the relative expressions. β -actin was used as the internal control. ΔΔCt = [ΔCt (Ct value of target gene in experiment group-Ct value of β -actin)-ΔCt (Ct value of target gene in control group- Ct value of β -actin)]. Each group repeats five independent samples, and each independent sample repeats three times to take the mean value. One way ANOVA/Tukey was used for statistical analysis. All results were mean ± SEM. * p < 0.05. (E) – (H) The protein expression of IL-4, IL-5, IL-13, and IL-9 in nasal mucosa of each group which was detected by flow cytometry bead array. Each group repeats five independent samples. One way ANOVA/Tukey was used for statistical analysis. All results were mean ± SEM. * p < 0.05.

    Article Snippet: The flow cytometry bead array flex Kit (BD Biosciences, San Diego, California, United States) was used according to the manufacturer’s instructions to detect IL-4, IL-5, IL-9, and IL-13 levels in the supernatant.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Flow Cytometry

    Schematic diagram and statistical diagram of flow cytometry. Figure (A) – (C) shows the Th2 delineation strategy of the control group, and other groups follow this scheme. Figure (D) shows the average number of Th2 cells in each group ( n = 5). One way ANOVA/Tukey was used for statistical analysis. All results were mean ± SEM. * p < 0.05.

    Journal: Frontiers in Pharmacology

    Article Title: IL-9 neutralizing antibody suppresses allergic inflammation in ovalbumin-induced allergic rhinitis mouse model

    doi: 10.3389/fphar.2022.935943

    Figure Lengend Snippet: Schematic diagram and statistical diagram of flow cytometry. Figure (A) – (C) shows the Th2 delineation strategy of the control group, and other groups follow this scheme. Figure (D) shows the average number of Th2 cells in each group ( n = 5). One way ANOVA/Tukey was used for statistical analysis. All results were mean ± SEM. * p < 0.05.

    Article Snippet: The flow cytometry bead array flex Kit (BD Biosciences, San Diego, California, United States) was used according to the manufacturer’s instructions to detect IL-4, IL-5, IL-9, and IL-13 levels in the supernatant.

    Techniques: Flow Cytometry